Andrew I Shevchuk; Pavel Novak; Yasufumi Takahashi; Richard Clarke; Michele Miragoli; Babak Babakinejad; Julia Gorelik; Yuri E Korchev; David Klenerman
DisclosuresNanomedicine. 2011;6(3):565-575.
Andrew I Shevchuk1, Pavel Novak1,2, Yasufumi Takahashi1, Richard Clarke3, Michele Miragoli2, Babak Babakinejad1, Julia Gorelik2, Yuri E Korchev1 & David Klenerman†3
1Division of Medicine, Imperial College London, London W12 0NN, UK
2National Heart & Lung Institute, Department of Cardiac Medicine, Imperial College London, London SW3 6LY, UK
3Department of Chemistry, Cambridge University, Cambridge CB2 1EW, UK
†Author for correspondence
Tel.: +44 1223 336 481 Fax: +44 1223 336 362 dk10012@cam.ac.uk
Ion conductance microscopy can scan complex cellular structures and tissues with nanoscale resolution.
A suite of different imaging modalities can be performed with the same instrument in order to image cell function at the same time. This includes fluorescence, mapping chemical species and the controlled delivery of molecules and will continue to be extended by fuctionalization of the nanopipette probe.
Experiments on the localization of β adrenergic receptors with cAMP signaling on heart cells illustrate how these methods can provide new insights into how normal cells are organized and how this changes during disease.
Single molecule physiology should be possible in the next few years, allowing the function of single receptors to be studied in the membrane of live cells.
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