Applying Selected Reaction Monitoring to Targeted Proteomics

Enrique Calvo; Emilio Camafeita; Benjamín Fernández-Gutiérrez; Juan A López

Disclosures

Expert Rev Proteomics. 2011;8(2):165-173. 

In This Article

Concluding Remarks

Although SRM is not exempt from problems, several of the obstacles pointed out could be solved with a careful selection of the precursor and diagnostic ions, as well as of the chromatographic and spectrometric conditions. Thus, hundreds of transitions can be monitored simultaneously when using scheduled SRM assays optimized for the RT of each precursor. In addition to the selective quantification of low-abundant proteins in complex mixtures across multiple samples, SRM has great potential for the reliable identification and quantification of PTMs and other chemical modifications. Its outstanding multiplexing abilities, reproducibility, sensitivity and selectivity make SRM an invaluable tool in targeted proteomics and biomarker validation, with promising applications in medical screening.

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