Concluding Remarks
Although SRM is not exempt from problems, several of the obstacles pointed out could be solved with a careful selection of the precursor and diagnostic ions, as well as of the chromatographic and spectrometric conditions. Thus, hundreds of transitions can be monitored simultaneously when using scheduled SRM assays optimized for the RT of each precursor. In addition to the selective quantification of low-abundant proteins in complex mixtures across multiple samples, SRM has great potential for the reliable identification and quantification of PTMs and other chemical modifications. Its outstanding multiplexing abilities, reproducibility, sensitivity and selectivity make SRM an invaluable tool in targeted proteomics and biomarker validation, with promising applications in medical screening.
Expert Rev Proteomics. 2011;8(2):165-173. © 2011 Expert Reviews Ltd.
Cite this: Applying Selected Reaction Monitoring to Targeted Proteomics - Medscape - Apr 01, 2011.
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