Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time-including a large proportion of metabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.Copyright © 2021 Elsevier Ltd. All rights reserved.
About The Expert
Fiona S L Brinkman
Geoffrey L Winsor
Rachel E Done
Alain Filloux
Vanessa I Francis
Joanna B Goldberg
E Peter Greenberg
Kook Han
Robert E W Hancock
Cara H Haney
Susanne Häußler
Jens Klockgether
Iain L Lamont
Roger C Levesque
Stephen Lory
Pablo I Nikel
Steven L Porter
Matthew W Scurlock
Herbert P Schweizer
Burkhard Tümmler
Meng Wang
Martin Welch
References
PubMed